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IIP-Jagiellonian University in Krakow: Faculty of Biochemistry, Biophysics and Biotechnology
Krakow, Poland (Outgoing Program)
Program Terms:
Program Terms: Summer
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This program is currently not accepting applications.
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Fact Sheet: - unrelated header
Fact Sheet:
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Dept Offering Program: International Internship Program (IIP) Program Type: Internship
Program Features: Lab Based Work Time Away: Summer
Housing options: Student Responsibilty with support from IIP and/or Host Organization Program Group: International Internship Program
Duration of Program: 8 or more weeks Program Adviser: Shahreen Rahman
Program Description:
Program Description:
Jagiellonian University in Krakow

Organization Overview
The Faculty (a group of university departments) of Biochemistry, Biophysics and Biotechnology is one of the sixteen faculties of Jagiellonian University in Krakow. FBBB is a leading research and teaching institution in Poland well recognized internationally in the field of modern biology. The Faculty consists of 17 Departments/Laboratories and employs about 150 members of the academic staff. Research conducted at the Faculty includes contemporary biochemical, biophysical and biotechnological aspects of biological objects ranging from the molecular level through the cellular and up to the level of whole animal and plant organisms. Many modern experimental and computational methods are used by researchers of the Faculty in standard and specialized laboratories that are well equipped with modern scientific instruments. According to the Nature Index (May 2016), that tracks the affiliations of original research articles, the Faculty received the highest score among all life science research institutions in Poland. The Faculty has established broad international collaboration. Many scientists from all over the world visit Faculty each year giving lectures and participating in joint research programs. 

Intern Responsibilities:

1. Physical Biochemistry Intern
The Use of Fluorescence Techniques to Study the Interaction of CAP Protein With Small Molecule Ligands.
The cyclic AMP receptor protein (CAP) plays an important role in( recAMP). Upon binding the protein can bind to a specific DNA sequence and interact with RNA polymerase. CAP protein dimer has the ability to interact with up to 4 cAMP molecules, but it is not known which cAMP sites must be occupied to activate DNA binding. 
The aim of the proposed research is to examine binding of cAMP in physiological concentration ranges, and to examine the effect of CAP-DNA interaction on this process.
During the internship, the IIP intern will express recombinant CAP protein in bacteria; conduct purification using chromatographic procedures; and determine the purity and activity of the final preparation. Fluorescence labeling of the protein will also be carried out. Fluorescence techniques using endogenous and exogenous fluorophores will be used to study the interaction of CAP with cAMP.

Qualifications:
IIP candidates should have academic interest in life sciences, an understanding of biologically relevant systems and how they can be understood by chemical methods. Experience in laboratory work is welcome, but no specific proficiency is required. Communication in the laboratory will be in English. 
Sophomores and Juniors are encouraged to apply.


2. General Biochemistry Intern
Role of MCPIP3 in Skin Homeostasis
MCPIP3 is an RNase regulating the levels of selected transcripts. Preliminary data suggest that MCPIP3 might be important in skin homeostasis.
The IIP interns will culture human cell lines (HaCaT cells) and treat them with veEVs) are released from mammalian cells including stem cells (MSCs) and participate in cell-to-cell communication. Since MSC-derived EVs carry several bioactive components that may be transferred between cells, they are interesting objects for regenerative medicine as new tools for tissue repair. However, appropriate phenotypic characterization is required for further studies of EV impact on target cells in vitro and in vivo.
 
Thus, the goal of this project and the intern’s main responsibility will be to characterize EV specimens derived from MSCs via novel instruments dedicated for nanoparticle analysis including high resolution and imaging flow cytometry. The study will allow to identify the MSC-EV specimens with appropriate, defined phenotype that will be further used for other experiments including in studying their pro-regenerative impact on target cardiac and endothelial cells in vitro. The IIP intern will have a unique opportunity to learn methodological approaches for biological nanoparticle analysis and get familiar with unique dedicated instrumentation.

Qualifications:
The IIP candidates have to be familiar with basic laboratory techniques including pipetting, centrifugation and preparing basic solutions. Communication in the laboratory will be in English.
Sophomores and Juniors are encouraged to apply.


3. Immunology Intern
Phenotypic Characterization of Extracellular Vesicles
Extracellular vesicles (EVs) are released from mammalian cells including stem cells (MSCs) and participate in cell-to-cell communication. Since MSC-derived EVs carry several bioactive components that may be transferred between cells, they are interesting objects for regenerative medicine as new tools for tissue repair. However, appropriate phenotypic characterization is required for further studies of EV impact on target cells in vitro and in vivo.
 
Thus, the goal of this project and the IIP intern’s main responsibility will be to characterize EV specimens derived from MSCs via novel instruments dedicated for nanoparticle analysis including high resolution and imaging flow cytometry. The study will allow to identify the MSC-EV specimens with appropriate, defined phenotype that will be further used for other experiments including in studying their pro-regenerative impact on target cardiac and endothelial cells in vitro. The intern will have a unique opportunity to learn methodological approaches for biological nanoparticle analysis and get familiar with unique dedicated instrumentation.

Qualifications:
The IIP candidates have to be familiar with basic laboratory techniques including pipetting, centrifugation and preparing basic solutions. Communication in the laboratory will be in English.
Sophomores and Juniors are encouraged to apply.
 

Dates / Deadlines: - unrelated header
Dates / Deadlines:
This program is not currently accepting applications. Please consult the sponsoring department's website for application open dates.
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This program is currently not accepting applications.