Programs > Brochure
IIP-University of South Bohemia, Faculty of Fisheries and Protection of Waters Institute of Complex System
Nové Hrady, Czech Republic (Outgoing Program)
|Partner Institution/Organization Homepage:||Click to visit|
|Restrictions:||Princeton applicants only|
|Dept Offering Program:||IIP, International Internship Program (IIP)||Program Type:||Internship|
|Language Prerequisite:||No||Program Features:||Lab Based Work, Research|
|Degree Level:||2 First year Ugrad, 3 Sophomore, 4 Junior||Time Away:||Summer|
|Housing options:||Student Responsibilty with support from IIP and/or Host Organization||Program Group:||International Internship Program|
University of South Bohemia, Faculty of Fisheries and Protection of Waters Institute of Complex System
About: This project is part of the Biological Engineering Group in the Institute of Physical Biology. Brief background on the Group's main project: Frontiers of biology lie now in exact evaluation of biological processes, whether the field is called Systems Biology, Biological Engineering, or another name. There is currently discrepancy between processes observed in living cells, for example by microscopy, and models that are based mainly on biochemical observations--that is, interactions of proteins and metabolites extracted from the cell culture and examined in the test tube. There are many examples of non-homogeneous behavior in cells that have essential functional meaning and may be crucial for discrimination between living and non-living matter. In contrast to the prevalent approach the Group examines the macroscopic properties of cells and uses a stochastic systems approach from control engineering for model building. For that a new mathematical method of point information contribution and point information entropy hsa been developed, which is implemented into software used for evaluation of processes in living cells.
Intern Responsibilities: IIP interns may choose from a few existing projects or projects that align with the intern’s own interests may be arranged. Projects will include work in one or more of the following areas:
- Reconstruction of the living cells using optical microscopy in transmitted light and atomic force microscopy- This project will help IIP interns become acquainted with such microscopy techniques like atomic force microscopy (AFM) and optical microscopy. IIP interns will prepare on their own samples with lving cells for AFM imaging. Partakers will have an opportunity to work in the force spectroscopy mode in order to get information about mechanical properties and topology of different cell lines and to work with an optical microscope to obtain information on the cell interior. Also, the IIP intern will be introduced to image processing techniques based on information entropy approach. The aim of this project is to cultivate and fix cells, get images, perform image processing and reconstruction.
- Testing microscopes and algorithms developed at the ICS, tracking organelles- Their lab has developed an algorithm for 3D modelling and tracking of cell organelles and their images by an optical system of a transmission light microscope. The goal of this project is to develop and use 3D polygraphy for printing mass models of these organelles and their images by the microscope and find the trajectory of organelles' movement. The project aim is to develop a method of 3D polygraphy for printing mass models of cell organelles and draw the trajectories of moveable organelles.
- Fish school behavior in five information dimensions- Fish behavior is a prototype for behavior of higher organism. Upon their analysis, numerous extrapolations are made for fields as distant as robotics and human psychology. In previous years the method of observation of fish school formation was finalized.This year the goal of the project is to perform of number of repetitive experiments using various types of labyrinths to demonstrate the testability of various types of models. This project aims to verify the method of experimental analysis of fish school behavior and to prepare data for a scientific publication.
Previous work experiences (in the words of the past IIP interns): Intern #1: I tested and calibrated microscope equipment to determine its optimal functional limits; analyzed data from these tests using the lab's computational analysis protocols; and made a final poster and presentation. Intern #2: I worked on protein crystallization and atomic force microscopy (AFM) of living cells, particularly osteosarcoma cells, strain MG-62, and mice fibroblasts, strain L929. With protein crystallization, I prepared lysozyme crystals by trying out two different methods -- sitting drop and microseeding -- and successfully yielded crystals. I tried to find a way to adhere the crystals to the microscope slide so the sample does not move when it is being scanned. I contacted with a professor from NASA in order to figure out what materials he used so that I can replicate his experiment where he successfully held the protein in place. With atomic force microscopy, I was trained on using the machinery and how the images can be used to determine biochemical processes that are occurring within the cell. This can be used to determine whether or not a cell is at risk of certain diseases, especially cancer. I also learned to cultivate cells and immobilize them for scanning by AFM. I was trained in using the machinery and using it to take elasticity measurements of the cell. I used this information to determine which cells were most compatible with which substrate, which helped determine what material something should be made out of for permanent insertion into the body. In the end, I presented my project on a powerpoint and created a poster for future displays.
View a PowerPoint presentation by a past intern:
Seshadri, Divya, University of South Bohemia, Czech Republic.pdf
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